VISUALIZATION AND COLONIZATION STUDIES OF A NOVEL BACTERIUM LACTOBACILLUS THERMOTOLERANS ON CHICKEN CECUM USING FLUORESCENCE IN SITU HYBRIDIZATION-CONFOCAL LASER SCANNING MICROSCOPE (FISH-CLSM) | | Author : ABU SADEQUE MD. SELIM* AND ATSUSHI YOKOTA | | Abstract | Full Text | Abstract :The direct visualization and colonization studies of Lactobacillus thermotolerans in cecal epithelium of chicken was attempted by applying fluorescence in situ hybridization in combination with confocal laser scanning microscope (FISH-CLSM) method. Chicken cecum (0.5 cm) as collected from White Leghorn were fixed, washed and then embedded in Tissue-Tek OCT compound. Vertical thin section (20 mm) of the fixed samples was prepared using Cryostat-3000, Leyca. Then the slices were fixed on gelatin-coated microscopic slides, air dried, and dehydrated in a series of increasing concentrations of ethanol followed by fluorescence in situ hybridization (FISH) and observed by confocal laser scanning microscope (CLSM). CLSM confirmed that bacteria were located in layers of the cecum tissue. Accordingly, the FISH technique demonstrated that the bacteria consist of cocci. The hybridized cells of target strain were found within the total bacteria on cecum surface, the results clearly demonstrated that L. thermotolerans are the inhabitants of chicken microbiota and colonized at a low number in terms of total bacteria. |
| EFFECT OF DIETARY INCLUSION OF WHOLE SUNFLOWER SEEDS ON FEEDING LACTATING ZARAIBI GOATS: MILK PRODUCTION AND COMPOSITION AS WELL AS MAMMARY GLAND HISTOLOGY AND ECONOMIC EFFICIENCY | | Author : ABDELHAMID, A.M, T.A.M. ASHMAWY*, FATEN F. ABOU AMMOU AND HEBA A. EL-SANAFAWY | | Abstract | Full Text | Abstract : The present study aimed to evaluate the effect of replacing concentrate feed mixture containing cotton seed cake by 15 or 20% of whole sunflower seeds on milk production and composition as well as economic efficiency of does. A total of 30 local breed (Zaraibi) goat does from 4-5 years of age, with an average of 42.46±0.86 kg LBW and within 3 parity were used in this study. Does were fed a basal ration consisting of 25% concentrate feed mixture (CFM) beside 75% fresh berseem during winter feeding or 50% CFM and 50% berseem hay (BH) during summer feeding. During the last month of pregnancy, all does were divided into three groups (10 does each). Goats in the 1st group (G1) served as a control and were fed the basal ration (Control). However, does in the 2nd (G2) and 3rd (G3) groups were fed the control ration, but 15 and 20% of CFM were replaced by whole sunflower seeds, respectively. Average daily milk yield during the suckling period was the highest |
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